Journal: Cell

Article Title: Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory

doi: 10.1016/j.cell.2019.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Prior imaging, sample were mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Fisher Scientific, cat. NC9524612).

Techniques: Western Blot, Flow Cytometry, Control, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Recombinant, SYBR Green Assay, cDNA Synthesis, Red Blood Cell Lysis, Software

Journal: Diseases

Article Title: Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity

doi: 10.3390/diseases11040124

Figure Lengend Snippet: Immunofluorescence staining for pro-oxidative redox enzymes in aortic tissue sections. Immunoreactivity to NOX1 was markedly elevated in the endothelium of ApoE−/− mice ( A ). Likewise, NOX2 immunoreactivity was pronounced in the ApoE−/− mouse endothelium ( B ). Interestingly, NOX4 immunoreactivity was not elevated in the endothelium, but in the media of ApoE−/− mice ( C ). Immunoreactivity to XDH/XO was weak in both wild-type and ApoE−/− mice ( D ). Data are presented as mean ± SE normalized to wild-type controls (n = 8 per genotype; *** p < 0.001; ** p < 0.01, ApoE−/− versus wild-type mice). The white arrows point to the endothelial layer. Blue: DAPI; Red: Rhodamine Red-X-coupled. Scale bar = 20 µm.

Article Snippet: Finally, the prepared slides were mounted using VECTASHIELD® Mounting Medium with DAPI (BIOZOL Diagnostica Vertrieb GmbH, Eching, Germany) and cover-slipped.

Techniques: Immunofluorescence, Staining

Journal: Diseases

Article Title: Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity

doi: 10.3390/diseases11040124

Figure Lengend Snippet: Immunofluorescence staining for pro-oxidative redox enzymes in ophthalmic artery cross-sections. Immunoreactivity to NOX1 ( A ), NOX2 ( B ), and NOX4 ( C ) was negligible in both wild-type and ApoE−/− mice. Only faint immunoreactivity to XDH/XO was visible in the media and adventitia of the ophthalmic artery but was similar in wild-type and ApoE−/− mice ( D ). Data are presented as mean ± SE normalized to wild-type controls (n = 8 per genotype). The white arrows point to the endothelial layer. Blue: DAPI; Red: Rhodamine Red-X-coupled. Scale bar = 20 µm.

Article Snippet: Finally, the prepared slides were mounted using VECTASHIELD® Mounting Medium with DAPI (BIOZOL Diagnostica Vertrieb GmbH, Eching, Germany) and cover-slipped.

Techniques: Immunofluorescence, Staining

Journal: Diseases

Article Title: Studies on the Effects of Hypercholesterolemia on Mouse Ophthalmic Artery Reactivity

doi: 10.3390/diseases11040124

Figure Lengend Snippet: LOX-1 immunoreactivity in the aorta and in the ophthalmic artery of wild-type and ApoE−/− mice. In the aorta from ApoE−/− mice, immunoreactivity to LOX-1 was increased in both the endothelium and the smooth muscle compared to wild-type mice ( A ). In contrast, LOX-1 staining was very faint in the endothelium of the ophthalmic artery from both mouse genotypes ( B ). Although pronounced LOX-1 immunoreactivity was observed in the ophthalmic artery smooth muscle, there was no difference in staining intensity between ApoE−/− and wild-type mice. Data are presented as mean ± SE normalized to wild-type controls (n = 8 per genotype; *** p < 0.001; ** p < 0.01, ApoE−/− versus wild-type mice). The white arrows point to the endothelial layer. Blue: DAPI; Red: Rhodamine Red-X-coupled. Scale bar = 20 µm.

Article Snippet: Finally, the prepared slides were mounted using VECTASHIELD® Mounting Medium with DAPI (BIOZOL Diagnostica Vertrieb GmbH, Eching, Germany) and cover-slipped.

Techniques: Staining